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Background  Desacetoxyvindoline-4-hydroxylase is a key enzyme in the biosynthesis of vindoline, the important intermediate leading to vinblastine and vincristine in  Catharanthus roseus .    Results  A  d4h-like  gene has been isolated from  C. roseus  C 20 hi cells based on an EST sequence from the Suppression Subtractive Hybridization cDNA library. The full length cDNA of  d4h-like  was 1427 bp encoding 372 amino acids. It had 66% identities and 80% positives with  d4h  at the amino acid level. It belonged to 2-oxoglutarate dependent oxygenase superfamily as  d4h  did. Real-time quantitative PCR analysis revealed that  d4h-like  was expressed high in roots, flowers and C 20 hi cells, very low in leaves and stems. Methyl jasmonate could significantly increase the accumulation of  d4h-like  transcripts. 2,4-D inhibited its expression. An approximate 2,910 bp of 5′-promoter region of  d4h-like  was obtained, fused to  GUS  reporter gene and analyzed with fluorescence quantitative assays using transient expression in  C. roseus  cell suspensions, indicating that  d4h-like  promoter could drive GUS gene expression in vivo.    Conclusion  These results suggest that  d4h-like  is closely related with  d4h  in the genetic evolution but with different transcriptional expression profiles. It may be revolved in the hormone-independency of C 20 hi cells.    Electronic supplementary material  The online version of this article (doi:10.1186/1999-3110-55-29) contains supplementary material, which is available to authorized users.

An active Catharanthus roseus desacetoxyvindoline-4-hydroxylase-like gene and its transcriptional regulatory profile