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Materials and methods Twelve newborn female Wistar rats were used, and half of them submitted to saline solution administration (control) and the other half were administrated monosodium glutamate solution, 4.0 mg/Kg body weight (obese) in neonatal period. At adult age (90 days of life) these female rats were mated with health male rats and the female offspring were used, divided into two groups: control (CONT, n=28) and obese (OB, n=15), according to its previous dam group. In all adult age (from 3rd to 7th months) the rats were monthly evaluate the Lee Index, water and food intake, 12h-fasting glycemia, oral glucose tolerance test (OGTT) and insulin test tolerance (ITT). In addition, from OGTT Results it was estimated the area under the glycemic curve (AUC). All data were statistically analyzed with 5% significance.

The transgenerational study of insulin action in female offspring adult Wistar rats