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Autofluorescence imaging has recently been shown to improve the detection of premalignant and malignant oral lesions. This method is based on the illumination in the absorption of tissue fluorophore molecules (NADH and FAD in the epithelial layer and collagen, and elastin in the stroma) in ultraviolet visible spectrum leading to the emission of lower energy photon that can be detected as fluorescence from the oral surface mucosa. Studies of these methods in normal oral mucosa have shown increased green fluorescence in comparison to neoplastic lesions upon ultraviolet (UV) or near UV light source. The histopathologic manifestations and heterogeneity of oral squamous lesions and the confounding factors for the validation and the clinical applications of autofluoresence imaging will be presented and discussed. from 1st Scientific Meeting of the Head and Neck Optical Diagnostics Society

Role of histopathologic and phenotypic assessment in the development and validation of optical diagnostic devices for head and neck mucosal lesions