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Methods HIV-1 genotypic resistance was determined from 1,327 treated patients failing on the first treatment regimen (HIV-RNA >1000 copies/mL) later than 1998. All sequences along with a detailed treatment history record were stored in a HIV sequence database. Potential association between the levels of resistance and the percentage of drugs received before resistance testing per year was explored by estimating the slope of change in the proportion of resistant strains over increasing drug use.

Correlation between patterns of HIV-1 drug resistance and drug administration in antiretroviral experienced patients in Greece during 1999–2006