English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background   Campylobacter jejuni , a gram-negative bacterium, is a frequent cause of gastrointestinal food-borne illness in humans throughout the world. There are several reports that the virulence of  C. jejuni  might be modulated by non-flagellar proteins that are secreted through the filament. Recently, FspA (Flagella secreted proteins) have been described. Two alleles of  fspA  ( fspA1  and  fspA2 ) based on sequence analysis were previously reported and only the  fspA2  allele was found in Thai isolates. The aim of this study is to analyze the deduced amino acid sequences  fspA  and the adjacent putative integral membrane protein from 103 Thai  C. jejuni  isolates.    Results  A total of 103 representative  C. jejuni  isolates were amplified by PCR for the  fspA  gene and the adjacent integral membrane protein gene. Two PCR product sizes were amplified using the same primers, an approximately 1600-bp PCR product from 19 strains that contained  fspA  and integral membrane protein genes and an approximately 800-bp PCR product from 84 strains that contained only the  fspA  gene. DNA sequencing was performed on the amplified products. The deduced amino acid sequences of both genes were analyzed separately using CLC Free Workbench 4 software. The analysis revealed three groups of FspA. Only FspA group 1 sequences (19/103) (corresponding to  fspA1 ) consisting of 5 subgroups were associated with the adjacent gene encoding the integral membrane protein. FspA group 2 was the largest group (67/103) consisting of 9 subgroups. FspA group 2p (17/103) consisting of 7 subgroups was found to contain stop codons at a position before the terminal 142 position.    Conclusions  This study reveals greater heterogeneity of FspA (group 1, 2 and 2p) among Thai  C. jejuni  isolates than previously reported. Furthermore, the subgroups of FspA groups 1 were associated with groups of integral membrane protein. The significance of these different FspA variants to virulence requires further study.

Distribution of flagella secreted protein and integral membrane protein among Campylobacter jejuni isolated from Thailand