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Background  The objective of this study was to build the transcriptomic profile of granulosa cells originating from follicles 6 to 9 mm in diameter in dairy cattle using microarrays.    Methods  Granulosa cells originating from three different phases of antral follicle growth were compared: growing (G), plateau (P) and atresia (A), as categorized by flow cytometry profiles of DNA. The growing and atretic conditions were each hybridized against the plateau condition as a reference in order to understand the specific biological mechanisms modulated in this class of follicles.    Results  2,942 genes were differentially expressed (P < 0.05) in P vs. G and 1,974 in A vs. P. A clear segregation of the 3 phases was confirmed by between group analysis (BGA). The first characteristic of the plateau phase is the activation of the upstream regulators  TP53  and  PTEN  which participate in the reduction of cell growth through  MYC ,  FOS  and  E2F1-2-3.  We also observed the down-regulation of steroidogenesis genes:  CYP11A1  and  CYP19A1 , in the granulosa cells of the plateau phase relative to the growth phase. On the other hand, the A vs. P contrast showed up-regulation of multiple transcripts associated to apoptosis:  CCT2, DAB2, DSG2  and  TGM2 .    Conclusions  This study offers multiple candidate genes to be further studied in order to elucidate their role in the modulation of follicular development and, ultimately, of oocyte quality.

Changes in granulosa cells gene expression associated with growth, plateau and atretic phases in medium bovine follicles