English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Apicomplexan parasites of the genus  Babesia ,  Theileria  and  Plasmodium  are very closely related organisms. Interestingly, their mitochondrial (mt) genomes are highly divergent. Among  Babesia ,  Babesia orientalis  is a new species recently identified and specifically epidemic to the southern part of China, causing severe disease to water buffalo. However, no information on the mt genome of  B. orientalis  was available.    Methods  Four pairs of primers were designed based on the full genome sequence of  B. orientalis  (unpublished data) and by aligning reported mt genomes of  B. bovis ,  B. bigemina , and  T. parva . The entire mt genome was amplified by four sets of PCR. The obtained mt genome was annotated by aligning with published apicomplexan mt genomes and Artemis software v11. Phylogenetic analysis was performed by using  cox1  and  cob  amino acid sequences.    Results  The complete mt genome of  B. orientalis  (Wuhan strain) was sequenced and characterized. The entire mt genome is 5996 bp in length with a linear form, containing three protein-coding genes including cytochrome  c  oxidase I ( cox1 ), cytochrome b ( cob ) and cytochrome  c  oxidase III ( cox3 ) and six rRNA large subunit gene fragments. The gene arrangement in  B. orientalis  mt genome is similar to those of  B. bovis ,  B. gibsoni  and  Theileria parva , but different from those of  T. orientalis ,  T. equi  and  Plasmodium falciparum . Comparative analysis indicated that  cox1  and  cob  genes were more conserved than  cox3 . Phylogenetic analysis based on amino acid sequences of  cox1 ,  cob  and  cox1  +  cob , respectively, revealed that  B. orientalis  fell into  Babesia  clade with the closest relationship to  B. bovis .    Conclusions  The availability of the entire mt genome sequences of  B. orientalis  provides valuable information for future phylogenetic, population genetics and molecular epidemiological studies of apicomplexan parasites.

Mitochondrial genome of Babesia orientalis, apicomplexan parasite of water buffalo (Bubalus babalis, Linnaeus, 1758) endemic in China