English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Considering the increasing importance of small animals travel medicine and the spread of filariae with zoonotic potential to non-endemic European areas, routine filarial diagnosis in dogs is becoming important.  Dirofilaria immitis ,  D. repens ,  Acanthocheilonema dracunculoides  and  A. reconditum  are the most common canine filarial nematodes presenting blood circulating microfilariae (mf) which can be differentiated to species level by the acid phosphatase activity patterns or by PCR. Available data on the size of the mf vary considerably in the literature. The aim of this study was to validate morphometric criteria for filarial identification in blood samples of dogs after concentration of mf with the modified Knott’s technique.    Methods  Morphometric analysis of 10 mf from samples identified to species level by acid phosphatase activity and partially confirmed by PCR were performed with specimens from 377 dogs.    Results  The mean length and width of  D. immitis  mf from 60 dogs were 301.77±6.29 μm and 6.30±0.26 μm, of  D. repens  mf from 171 dogs 369.44±10.76 μm 8.87±0.58 μm, of  A. dracunculoides  mf from 133 dogs 259.43±6.69 μm and 5.09±0.47 μm and of  A. reconditum  mf from 13 dogs 264.83±5.47 μm and 4.63±0.52 μm.  For a subset of 30 samples, morphometric analysis was repeated with identical results in two laboratories. Furthermore, the size of mf concentrated and fixed by the Knott’s technique was shown to be stable over 105 days.    Conclusions  The Knott’s test enables to clearly distinguish between  D. immitis ,  D. repens  and  Acanthocheilonema  spp. However, due to the overlapping size ranges of  A. dracunculoides  and  A. reconditum , biochemical or molecular methods are required to distinguish these two species.

parasites and vectors

Morphometric analyses of canine blood microfilariae isolated by the Knott’s test enables Dirofilaria immitis and D. repens species-specific and Acanthocheilonema (syn. Dipetalonema) genus-specific diagnosis