English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Anaplasma phagocytophilum  is an emerging pathogen of humans, dogs and other animals, and it is transmitted by ixodid ticks. The objective of the current study was a) detect  A. phagocytophilum  in dogs and ixodid ticks using real-time Polymerase Chain Reaction (qPCR); and b) Determine important variables associated to host, environment and potential tick vectors that are related to the presence of  A. phagocytophilum  in dogs domiciled in Rio de Janeiro, Brazil.    Methods  We tested blood samples from 398 dogs and samples from 235 ticks, including 194  Rhipicephalus sanguineus  sensu lato, 15  Amblyomma cajennense , 8  Amblyomma ovale  and 18 pools of  Amblyomma  sp. nymphs. A semi-structured questionnaire was applied by interviewing each dog owner. Deoxyribonucleic acid obtained from ticks and dog buffy coat samples were amplified by qPCR ( msp2  gene). The sequencing of  16S rRNA  and  groESL  heat shock operon genes and a phylogenetic analysis was performed. The multiple logistic regression model was created as a function of testing positive dogs for  A. phagocytophilum .    Results  Among the 398 blood samples from dogs, 6.03% were positive for  A. phagocytophilum. Anaplasma phagocytophilum  was detected in one  A. cajennense  female tick and in five  R. sanguineus  sensu lato ticks (four males and one female). The partial sequences of the  16S rRNA , and  groESL  genes obtained were highly similar to strains of  A. phagocytophilum  isolated from wild birds from Brazil and human pathogenic strains. The tick species collected in positive dogs were  R. sanguineus  sensu lato and  A. cajennense , with  A.cajennense  being predominant. Tick infestation history (OR = 2.86, CI = 1.98-14.87), dog size (OR = 2.41, IC: 1.51-12.67), the access to forest areas (OR = 3:51, CI: 1.52-16.32), hygiene conditions of the environment in which the dogs lived (OR = 4.35, CI: 1.86-18.63) and  Amblyomma  sp. infestation (OR = 6.12; CI: 2.11-28.15) were associated with  A. phagocytophilum  infection in dogs.    Conclusions  This is the first report of  A. phagocytophilum  in ixodid ticks from Brazil. The detection of  A. phagocitophylum  in  A. cajennense , an aggressive feeder on a wide variety of hosts, including humans, is considered a public health concern.

Molecular epidemiology of the emerging zoonosis agent Anaplasma phagocytophilum (Foggie, 1949) in dogs and ixodid ticks in Brazil