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Background   Ehrlichia  species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious and fatal infections in companion animals and livestock. The aim of this paper was to phylogeneticaly characterise a new species of  Ehrlichia  isolated from  Rhipicephalus (Boophilus) microplus  from Minas Gerais, Brazil.    Methods  The agent was isolated from the hemolymph of  Rhipicephalus (B.) microplus  engorged females that had been collected from naturally infested cattle in a farm in the state of Minas Gerais, Brazil. This agent was then established and cultured in IDE8 tick cells. The molecular and phylogenetic analysis was based on  16S rRNA ,  groEL, dsb ,  gltA  and  gp36  genes. We used the maximum likelihood method to construct the phylogenetic trees.    Results  The phylogenetic trees based on  16S rRNA ,  groEL, dsb  and  gltA  showed that the  Ehrlichia  spp isolated in this study falls in a clade separated from any previously reported  Ehrlichia  spp. The molecular analysis of the ortholog of  gp36,  the major immunoreactive glycoproteins in  E. canis  and ortholog of the  E. chaffeensis gp47,  showed a unique tandem repeat of 9 amino acids (VPAASGDAQ) when compared with those reported for  E. canis ,  E. chaffeensis  and the related mucin-like protein in  E. ruminantium .    Conclusions  Based on the molecular and phylogenetic analysis of the  16S rRNA ,  groEL, dsb  and  gltA  genes we concluded that this tick-derived microorganism isolated in Brazil is a new species, named  E. mineirensis  (UFMG-EV), with predicted novel antigenic properties in the  gp36  ortholog glycoprotein. Further studies on this new  Ehrlichia  spp should address questions about its transmissibility by ticks and its pathogenicity for mammalian hosts.

parasites and vectors

New species of Ehrlichia isolated from Rhipicephalus (Boophilus) microplus shows an ortholog of the E. canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats