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Background  Cathepsin cysteine proteases play multiple roles in the life cycle of parasites such as food uptake, immune invasion and pathogenesis, making them valuable targets for diagnostic assays, vaccines and drugs. The purpose of this study was to identify a cathepsin B of  Clonorchis sinensis  ( Cs CB) and to investigate its diagnostic value for human helminthiases.    Results  The predicted amino acid sequence of the cathepsin B of  C. sinensis  shared 63%, 52%, 50% identity with that of  Schistosoma japonicum ,  Homo sapiens  and  Fasciola hepatica , respectively. Sequence encoding proenzyme of  Cs CB was overexpressed in  Escherichia coli . Reverse transcription PCR experiments revealed that  Cs CB transcribed in both adult worm and metacercaria of  C. sinensis .  Cs CB was identified as a  C. sinensis  excretory/secretory product by immunoblot assay, which was consistent with immunohistochemical localization showing that  Cs CB was especially expressed in the intestine of  C. sinensis  adults. Both ELISA and western blotting analysis showed recombinant  Cs CB could react with human sera from clonorchiasis and other helminthiases.    Conclusions  Our findings revealed that secreted CsCB may play an important role in the biology of C. sinensis and could be a diagnostic candidate for helminthiases.

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Molecular characterization of cathepsin B from Clonorchis sinensis excretory/secretory products and assessment of its potential for serodiagnosis of clonorchiasis