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Background  Heat stress leads to accelerated production of reactive oxygen species (ROS) which causes a huge amount of oxidative damage to the cellular components of plants. A large number of heat stress related genes as HSPs, catalases, peroxidases are overexpressed at the time of stress. A potent stress responsive gene peroxisomal ascorbate peroxidase ( TapAPX ) obtained from heat stress (42°C) responsive subtractive cDNA library from a thermo tolerant wheat cv. Raj3765 at anthesis stage was cloned, characterized and its role was validated under heat stress by proteomics and  in-silico  studies. In the present study we report the characterization at molecular and  in-silico  level of peroxisomal  TapAPX  gene isolated from heat tolerant wheat cultivar of India.    Results  qPCR studies of  TapAPX  gene displayed up to 203 fold level of expression at 42°C heat stress exposure. A full length cDNA of 876 bp obtained by RACE deduced a protein of 292 amino acid residues which gives a complete 3D structure of  pAPX  by homology modeling.  TapAPX  cDNA was cloned in expression vector pET28 (a+) and the recombinant protein over-expressed in  E. coli  BL21 showed highest homology with APX protein as deduced by peptide mass fingerprinting.    Conclusions   TapAPX  gene from wheat cv Raj3765 has a distinct role in conferring thermo tolerance to the plants and thus can be used in crop improvement programmes for development of crops tolerant to high temperature.    Electronic supplementary material  The online version of this article (doi:10.1186/1756-0500-7-713) contains supplementary material, which is available to authorized users.

Molecular cloning and in-silico characterization of high temperature stress responsive pAPX gene isolated from heat tolerant Indian wheat cv. Raj 3765