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Background  Cyclophilins (CyP), conserved in all genera, are known to have regulatory responses of various cellular processes including stress tolerance. Interestingly, CyP has a crucial role as peptidyl-prolyl  cis – trans  isomerases (PPIases). Our earlier  in silico  based approach resulted into the identification of cyclophilin family from rice,  Arabidopsis  and yeast. In our recent report, we discovered a new OsCYP-25 from rice. Here, we identified a novel cyclophylin A-like protein (PiCyP) from  Piriformospora indica  which is responsible for abiotic stress tolerance in  E. coli .    Results  Cyclophylin A-like protein (CyPA) (accession number GQ214003) was selected from cDNA library. The genomic organization CyPA revealed a 1304 bp of  CyPA  in  P. indica  genome, showing 10 exons and 9 introns. Further,  CyPA  was evident in PCR with gDNA and cDNA and Southern blot analysis. The phylogenetic examination of CyPA of  P. indica  showed that it is closed to human cyclophilin. The uniqueness of PiCyPA protein was apparent in western blot study. Kinetics of purified PiCyPA protein for its PPIas activity was determined via first order rate constant (0.104 s -1 ) in the presence of 1 μg of PiCyPA, with increasing PiCyPA concentration, in the presence of cyclosporin A (CsA) and the inhibition constant (4.435 nM) of CsA for inhibition of PiCyPA. The differential response of  E. coli  harbouring pET28a- PiCypA  was observed for their different degree of tolerance to different abiotic stresses as compared to empty pET28a vector.    Conclusions  Overexpression of PiCyPA protein  E. coli  cells confer enhanced tolerance to wide range of abiotic stresses. Thus, this study provides the significance of PiCypA as a molecular chaperone which advanced cellular stress responses of  E. coli  cells under adverse conditions, and it, furthermore, confirms the mounting the sustainability of  E. coli  for exploitation in recombinant proteins production. Additionally, the  PiCyPA  gene cooperates substantial functions in cellular network of stress tolerance mechanism, essentially required for various developmental stages, and might be a potential paramount candidate for crop improvement and its sustainable production under adverse conditions.

Molecular characterization of cyclophilin A-like protein from Piriformospora indica for its potential role to abiotic stress tolerance in E. coli