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Background   Duddingtonia flagrans  and  Monacrosporium thaumasium  are promising fungus species in veterinary biological control of gastrointestinal nematodes because of their production capacity of fungal structures (conidia and/or chlamydospores), growth efficiency in laboratory solid media and especially their predatory capacity. However, their large-scale production remains a challenge. This work aimed at evaluating the mycelial mass production of  D .  flagrans  (AC001 and CG722) and  M .  thaumasium  (NF34A) nematophagous fungi under different culture conditions.    Results  The results did not present significant differences (p > 0.05) in mycelia mass production between the isolates cultured under pH 4.0. Furthermore, after 168 hrs., the isolate CG722 presented a lower production of mycelial mass in medium CM (corn meal) (p < 0.05).    Conclusion  We therefore concluded the use of culture media SD (soy dextrose) and CG (corn grits) at pH values between 6.0 and 7.0 is suitable for high mycelial mass production of  D .  flagrans  and  M .  thaumasium .

Mycelial mass production of fungi Duddingtonia flagrans and Monacrosporium thaumasium under different culture conditions