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Background  Rough lemon ( Citrus jambhiri  Lush.) is the most commonly used  Citrus  rootstock in south Asia. It is extremely sensitive to salt stress that decreases the growth and yield of  Citrus  crops in many areas worldwide. Over expression of the yeast halotolerant gene ( HAL2)  results in increasing the level of salt tolerance in transgenic plants.    Results  Transformation of rough lemon was carried out by using  Agrobacterium tumefaciens  strains LBA4404 harboring plasmid pJRM17. Transgenic shoots were selected on kanamycin 100 mg L -1  along with 250 mg L -1  each of cefotaxime and vancomycin for effective inhibition of  Agrobacterium  growth. The Murashige and Skoog (MS) medium containing 200 μM acetoseryngone (AS) proved to be the best inoculation and co-cultivation medium for transformation. MS medium supplemented with 3 mg L -1  of 6-benzylaminopurine (BA) showed maximum regeneration efficiency of the transformed explants. The final selection of the transformed plants was made on the basis of PCR and Southern blot analysis.    Conclusion  Rough lemon has been successfully transformed via  Agrobacterium tumefaciens  with β-glucuronidase ( GUS)  and  HAL2.  Various factors affecting gene transformation and regeneration efficiency were also investigated.

Agrobacterium-mediated transformation of rough lemon (Citrus jambhiri Lush) with yeast HAL2 gene