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Findings  To facilitate automation, a novel DNA extraction method for MRSA was adopted. The MRSA specific chromosome-SCC mec  PCR was adapted, additional primers were added, and the performance was validated. From various laboratories in The Netherlands we received a total of 86 MRSA clinical isolates, that were negative in commercially available tests. We identified 14 MRSA strains with new variant chromosome-SCC mec  junctions by sequence analysis. These MRSA strains appeared to carry SCC mec  sequences with a high degree of homology to SCC regions of  S. epidermidis  and  S. haemolyticus . All were included for detection in chromosome-SCC mec  based PCR.    Background  Efficient management of Methicillin Resistant  Staphylococcus aureus  (MRSA) in the hospital is needed to prevent dissemination. It is important that MRSA can be rapidly identified, and effective infection control measures can be initiated. Equally important is a rapid MRSA negative report, especially for patients in isolation. For negative screening we implemented fully automated high through-put molecular screening for MRSA.    Conclusions  Fourteen variant chromosome-SCC mec  junctions in MRSA, that are not detected in commercially available MRSA detection kits were added to our PCR to detect all currently known variant SCC- mec  types of MRSA.

Detection of novel chromosome-SCCmec variants in Methicillin Resistant Staphylococcus aureus and their inclusion in PCR based screening