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Background  In Dutch laboratories molecular detection of  B. pertussis  and  B. parapertussis  is commonly based on insertion sequences  IS 481 and  IS 1001, respectively. Both IS elements are more widely spread among  Bordetella  species. Both  Bordetella   holmesii , and  B. bronchiseptica  can harbour  IS 481. Also,  IS 1001 is found among  B. bronchiseptica .  IS 481, and  IS 1001 based PCR thus lacks specificity when used for detection of specific  Bordetella spp .    Findings  We designed a PCR based on  IS 1002, another IS element that is present among  Bordetella  species, and exploited it as a template in combination with PCR for  IS 481, and  IS 1001. In combining the PCRs for  IS 481,  IS 1001, and  IS 1002, and including an inhibition control, we were able to detect and discriminate all clinically relevant  Bordetella  species.    Conclusions  We developed an improved PCR method for specific detection of  B. pertussis ,  B. parapertussis, B. holmesii, and B. bronchiseptica.

bmc research notes

A real-time PCR assay with improved specificity for detection and discrimination of all clinically relevant Bordetella species by the presence and distribution of three Insertion Sequence elements