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Background  The gene coding for the uncharacterized protein PAB1135 in the archaeon  Pyrococcus abyssi  is in the same operon as the ribonuclease P (RNase P) subunit Rpp30.    Findings  Here we report the expression, purification and structural analysis of PAB1135. We analyzed the interaction of PAB1135 with RNA and show that it binds efficiently double-stranded RNAs in a non-sequence specific manner. We also performed molecular modeling of the PAB1135 structure using the crystal structure of the protein Af2318 from  Archaeoglobus fulgidus  ( 2OGK ) as the template.    Conclusions  Comparison of this model has lead to the identification of a region in PAB1135 that could be involved in recognizing double-stranded RNA.

Expression, purification and structural analysis of the Pyrococcus abyssi RNA binding protein PAB1135