English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Rust caused by  Puccinia psidii  Winter has been limiting for the establishment of new  Eucalyptus  plantations, as well as for resprouting of susceptible genetic materials. Identifying host genes involved in defense responses is important to elucidate resistance mechanisms. Reverse transcription-quantitative PCR is the most common method of mRNA quantitation for gene expression analysis. This method generally employs a reference gene as an internal control to normalize results. A good endogenous control transcript shows minimal variation due to experimental conditions.    Findings  We analyzed the expression of 13 genes to identify transcripts with minimal variation in leaves of 60-day-old clonal seedlings of two  Eucalyptus  clones (rust-resistant and susceptible) subjected to biotic ( P. psidii ) and abiotic (acibenzolar-S-methyl, ASM) stresses.    Conclusions  For tissue samples of clones that did not receive any stimulus, a combination of the  eEF2  and  EglDH  genes was the best control for normalization. When pathogen-inoculated and uninoculated plant samples were compared,  eEF2  and  UBQ  together were more appropriate as normalizers. In ASM-treated and untreated leaves of both clones, transcripts of the  CYP  and  elF4B  genes combined were the ones with minimal variation. Finally, when comparing expression in both clones for ASM-treated leaves,  P. psidii -inoculated leaves, ASM-treated plus  P. psidii -inoculated leaves, and their respective controls, the genes with the most stable expression were  EgIDH  and  UBQ . The chitinase gene, which is highly expressed in studies on plant resistance to phytopathogens, was used to confirm variation in gene expression due to the treatments.

bmc research notes

Selection of endogenous genes for gene expression studies in Eucalyptus under biotic (Puccinia psidii) and abiotic (acibenzolar-S-methyl) stresses using RT-qPCR