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Background  RNA-binding proteins of the PUF family share a conserved domain consisting of tandemly repeated 36-40 amino acid motifs (typically eight) known as Puf repeats. Proteins containing tandem repeats are often dominant targets of humoral responses during infectious diseases. Thus, we considered of interest to analyze whether  Leishmania  PUF proteins result antigenic during visceral leishmaniasis (VL).    Findings  Here, employing whole-genome databases, we report the composition, and structural features, of the PUF family in  Leishmania infantum . Additionally, the 10 genes of the  L. infantum  PUF family were cloned and used to express the  Leishmania  PUFs in bacteria as recombinant proteins. Finally, the antigenicity of these PUF proteins was evaluated by determining levels of specific antibodies in sera from experimentally infected hamsters. The  Leishmania  PUFs were all recognized by the sera, even though with different degree of reactivity and/or frequency of recognition. The reactivity of hamster sera against recombinant LiPUF1 and LiPUF2 was particularly prominent, and these proteins were subsequently assayed against sera from human patients. High antibody responses against rLiPUF1 and rLiPUF2 were found in sera from VL patients, but these proteins resulted also recognized by sera from Chagas' disease patients.    Conclusion  Our results suggest that  Leishmania  PUFs are targets of the humoral response during  L. infantum  infection and may represent candidates for serodiagnosis and/or vaccine reagents; however, it should be kept in mind the cross-reactivity of LiPUFs with antibodies induced against other trypanosomatids such as  Trypanosoma cruzi .

The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis