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Background  The Copper/Zinc superoxide dismutase (Cu/ZnSOD) gene,  SOD  gene, was isolated from a  Deschampsia antarctica  Desv. by cDNA library screening. The expression of SOD gene in the leaves of  D. antarctica  was determined by RT-PCR and its differential expression of gene transcripts in conditions of cold and UV radiation stresses was revealed by northern blot.    Findings  The molecular characterization shows that  SOD  cDNA is 709 bp in length, which translates an ORF of 152 amino acids that correspond to a protein of predicted molecular mass of 15 kDa. The assay shows that the expression of  SOD  gene increases when  D. antarctica  is acclimatised to 4°C and exposed to UV radiation. These results indicate that the  SOD  gene of  D. antarctica  is involved in the antioxidative process triggered by oxidative stress induced by the conditions of environmental change in which they live.    Conclusion  The present results allow us to know the characteristics of Cu/ZnSOD gene from  D. antarctica  and understand that its expression is regulated by cold and UV radiation.

bmc research notes

Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses