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Background  In the present pilot study we applied recently published protocols for detecting  Mycobacterium tuberculosis  in human remains. We screened long bones from an 18 th  century cemetery and skulls from the anatomical "Weisbach collection" (19 th  century). In addition, besides the study of abundance of tuberculosis in inmates of the poorhouse itself, we were interested to test whether in this particular instance tuberculosis can be identified from cortical bones, which are rarely affected by tuberculosis, but mostly better preserved than the vertebral bodies or epiphyses.    Method  The DNA extractions from the bone samples were obtained following established ancient DNA protocols. Subsequently extracts were subjected to a series of PCR amplifications using primer pairs published previously [ 1 , 2 ]. PCR products of the expected size were subsequently sequenced.    Results  Only primers targeting the repetitive IS 6110  insertion sequence yielded PCR products of appropriate size. In one sample only (skull sample WB354 of the "Weisbach collection") sequence analysis revealed an authentic  M. tuberculosis  sequence that matched to a reference sequence from GenBank.    Conclusion  With a variety of established PCR approaches we failed to detect  M. tuberculosis  DNA in historic human femurs from an 18 th  century cemetery relating to a poor house in Kaiserebersdorf, Austria. Our data may indicate that in this particular case, thoracic or lumbar vertebrae, i.e. bones that are severely affected by the disease, would be more suitable for molecular diagnostics than long bones. However, the unpredictable state of DNA preservation in bones from museum collections does not allow any general recommendation of any type of bone.

PCR diagnostics of Mycobacterium tuberculosis in historic human long bone remains from 18th century burials in Kaiserebersdorf, Austria