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Materials and methods The DNA was extracted from blood samples by using the standard phenol-chloroform extraction method and then amplified by PCR using specific primers for TPOX locus. The PCR products were separated by electrophoresis on denaturing 6% polyacrylamide gel and silver staining was done to resolve and observe different alleles. The observed and expected heterozygosity (Hobs and Hexp) as well as forensic and paternity indices including matching probability (MP), power of discrimination (PD), power of exclusion (PE) and polymorphism information content (PIC) were calculated using the Power Stats v.1.2 software for TPOX locus of the studied population. The POPGENE (v.32) statistical package was used for analyzing allele frequency.

Microsatellite variation and allele frequency distribution for (TPOX) STRS locus in North Indian Muslim populations