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Background  The C4 perennial grass  Miscanthus giganteus  has proved to be a promising bio-energy crop. However, the biomass recalcitrance is a major challenge in biofuel production. Effective pretreatment is necessary for achieving a high efficiency in converting the crop to fermentable sugars, and subsequently biofuels and other valued products.    Results   Miscanthus lutarioriparious  was pretreated with a liquid hot water (LHW) reactor. Between the pretreatment severity (PS) of 2.56-4.71, the solid recovery was reduced; cellulose recovery remained nearly unchanged; and the Klason lignin content was slightly increased which was mainly due to the dissolving of hemicellulose and the production of a small amount of pseudo-lignin. The result shows that a LHW PS of 4.71 could completely degrade the hemicellulose in  Miscanthus . Hemicellulose removal dislodged the enzymatic barrier of cellulose, and the ethanol conversion of 98.27% was obtained.    Conclusions  Our study demonstrated that LHW served as an effective pretreatment in case that  Miscanthus lutarioriparious  was used for ethanol production by simultaneous saccharification and fermentation. The combination and the pretreatment method of  Miscanthus  feedstock holds a great potential for biofuel production.

Pretreatment on Miscanthus lutarioriparious by liquid hot water for efficient ethanol production