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Background  Direct conversion of solar energy and carbon dioxide to drop in fuel molecules in a single biological system can be achieved from fatty acid-based biofuels such as fatty alcohols and alkanes. These molecules have similar properties to fossil fuels but can be produced by photosynthetic cyanobacteria.    Results   Synechocystis  sp. PCC6803 mutant strains containing either overexpression or deletion of the  slr1609  gene, which encodes an acyl-ACP synthetase (AAS), have been constructed. The complete segregation and deletion in all mutant strains was confirmed by PCR analysis. Blocking fatty acid activation by deleting  slr1609  gene in wild-type  Synechocystis  sp. PCC6803 led to a doubling of the amount of free fatty acids and a decrease of alkane production by up to 90 percent. Overexpression of  slr1609  gene in the wild-type  Synechocystis  sp. PCC6803 had no effect on the production of either free fatty acids or alkanes. Overexpression or deletion of  slr1609  gene in the  Synechocystis  sp. PCC6803 mutant strain with the capability of making fatty alcohols by genetically introducing fatty acyl-CoA reductase respectively enhanced or reduced fatty alcohol production by 60 percent.    Conclusions  Fatty acid activation functionalized by the  slr1609  gene is metabolically crucial for biosynthesis of fatty acid derivatives in  Synechocystis  sp. PCC6803. It is necessary but not sufficient for efficient production of alkanes. Fatty alcohol production can be significantly improved by the overexpression of  slr1609  gene.

Effects of fatty acid activation on photosynthetic production of fatty acid-based biofuels in Synechocystis sp. PCC6803