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Background Cancer/testis antigens is a group of proteins which expression is usually restricted to the human germ line but is also present at high levels in several types of cancer cells [1]. The antigen NY-ESO-1 is one of these antigens showing high immunogenicity and, therefore, holding great potential for a cancer vaccine [2]. Its heterologous expression is being tested in different expression systems, although the success has been limited [2,3]. In this report we used a transient expression system based on a PVX viral vector to evaluate the expression of NY-ESO-1 in leaves of Nicotiana benthamina.

Transient expression of the cancer/testis cancer antigen NY-ESO-1 in Nicotiana benthamiana using a PVX-based viral vector