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Background The turtles are usually associated with low substitution of individuals in the population because they have longer life than any other vertebrates. The order Testudines or Chelonia, the oldest among all vertebrates, comprising the turtles, terrestrial, marine and freshwater. These have as main characteristics, low growth rates and long periods required for mature, causing many turtles are vulnerable to extinction. Like other turtles, P. unifilis, popularly known as tracaja, leaves long-lived animals with late sexual maturity, characterized by a small mortality of adult animals, but high mortality rate of embryos and hatchlings, with natural predation as one of the most important factors of low hatching success of these species. This species is one of the most important representatives of the fauna of the Amazon turtle, but their eggs, meat and offal provide food for local communities, and have their hooves used as adornment and household items. The implementation of large hydropower enterprise cause many environmental damage. The scientific emphasis in EIA/RIMA should be held primarily in the study of special targets, and should take into account the genetic structure of populations. Mitochondrial molecular markers have particular maternal characteristics and high rates variation, which allow us to analyze the behavior of populations. The deployment of hydropower modifies the natural habitat of the turtles, which have difficulty in adapting. This work aimed to study the genetic structure of populations of P. unifilis analyzing the behavior of females in conditions of ambient modified by Tucurui, and Antonina-PA and Xambioa-TO, where will be built the dam of Santa Isabel. Methods We performed the sequencing of fragments of the control region of mitochondrial DNA, generating data of gene diversity and nucleotide, numbers of haplotypes and segregating sites, AMOVA, levels of structuring by pairwise comparison of FST and Mantel test in ARLEQUIN program. Clades were grouped checked by software Treefinder and the haplotype tree was held at hapview software.

Study of females of Podocnemis unifilis (Tracajá) (Troschel, 1848) in areas between Xambioá-TO, Antonina-PA and Tucuruí-PA, by molecular tools