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Background Different strategies have been evaluated for increasing the productivity of Drosophila melonogaster (S2) cells expressing recombinant rabies virus glycoprotein (RVGP). The maintenance of selective pressure during all the cultivation time and new methods for population enrichment with high level expressing cells are two methods which can increase the productivity through increased expression of RVGP gene. To understand how these methods can improve glycoprotein expression, analysis of the quantities of heterologous DNA and RNA were performed [1,2].

Influence of recombinant S2 cell population enrichment on rabies virus glycoprotein expression and especific RNA and DNA quantities