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Background Proteases are enzymes used in diversified process and industrial products, where the most important is the food sector and the detergent fabrication. The microbial obtention way is the most relevant, and the filamentous fungi are the main synthesis agents in industrial scale. [1]. The genres Conidiobolus, Verticillium, Penicillium e Aspergillus are reported as proteases producers. The specie Aspergillus oryzae is considerate not toxicogenic and it is a huge proteases producer, so it has an elevated industrial interest [2,3]. The literature about this theme is vast, there are countless articles using the Aspergillus in semi-solid fermentative processes to proteases of obtention. Nevertheless, the importance of this work was demonstrated by the use of an alternative substrate to protease synthesis, the canola meal. The canola meal it is a byproduct of the extraction process of canola oil, showing high protein content. Nowadays, the industrial sector has encouraged researches that can contribute and adding value to this residue [3]. So, in this context, this work proposed to evaluate preliminarily the performance of five lines of Aspergillus oryzae in relation to the production of proteases in semi-solid fermentation using the canola meal as a substrate inductor.

Production of proteolytic extract by Aspergillus oryzae grown by solid state fermentation using canola meal as substrate