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Background Bovine tuberculosis (TB) is a disease which constitutes an important economic and zoonotic problem in Latin America. The etiological agent, Mycobacterium bovis, has several antigens described and well characterized. The M. bovis antigen 85B (Ag85B), encoded by fbpB gene, is the bacillus immunodominant antigen and promising target for vaccine and diagnosis development [1]. Ag85B combined with other immunogens is considered as a potential diagnostic tool for TB ELISA assay [2]. Anti-Ag85B antibodies have high sensitivity and specificity for mycobacteria detection in commercial tests [3]. Here we describe the recombinant Ag85B production by Mycobacterium smegmatis, a fast growing mycobacterial specie.

Expression of recombinant Mycobacterium bovis antigen 85B by Mycobacterium smegmatis mc2