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Background The yeast strain CAT-1 isolated from a Brazilian fuelethanol plant (Babrzadeh et al. 2009) is one of the most common strain used nowadays due to its very efficient fermentation capacity, especially at high sugar concentrations and under the stressful industrial conditions. Since the transcription factor genes MSN4, MSN2, YAP1 and HSF1 of tolerant yeast strains are highly expressed under ethanol stress [1], we generated a CAT-1 derived strain named ATT-6 that overexpresses a truncated form of the transcription activator Msn2 through genomic engineering and analyzed the ethanol stress tolerance and fermentation capacity of this modified strain.

bmc proceedings

Overexpression of a truncated form of the MSN2 gene enhances the initial rate of ethanol production in an industrial fuel-ethanol Saccharomyces cerevisiae strain