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Nowadays, single-use bioreactors are widely accepted in pharmaceutical industry. This is based on shorter batch to batch times, reduced cleaning effort and a significantly lower risk of cross contaminations [1,2]. One large field of the application of single-use bioreactors is the seed train cultivation of mammalian cells [1]. The focus is further extended to perform state of the art fed-batch production processes in such bioreactors. In this study an industrial proven CHO fed-batch process is established in different single-use and reusable bioreactors.

The successful transfer of a modern CHO fed-batch process to different single-use bioreactors