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Materials and methods We used two human MDL tissue samples, three human MDL cell lines (UW473, UW472 and DAOY), three human cerebellum tissue samples and two human cerebellum primary culture. The cell lines were characterized morphologically by light microscopy and immunophenotypically by flow cytometry. MDL cell lines and tissue samples, as well as, control cerebellum primary culture and tissue were evaluated for gene expression profile of the EVF-2 lncRNA gene by Quantitative Real Time PCR.

Gene expression profile of long non-coding RNA EVF-2 in medulloblastoma cell lines and tissue samples