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Methods To gain insights into the mechanisms involved in breast cancer initiation and progression we conducted a miRNA global expression on 21T series. These cell lines represent an in vitro model of breast cancer progression comprising immortalized cell lines derived from the same patient diagnosed with Her-2 overexpressing ductal carcinoma. The set include a normal epithelia (16N), primary in situ ductal carcinoma (21PT and 21NT) and cells derived from pleural effusion of lung metastasis (21MT-1 and 21MT-2). To confirm microarray results, the expression of the most significantly altered miRNAs were checked by qPCR. Matrigel invasion assay was done to evaluate the migration capacity of 21Tcells and Her-2, ZEB-1 and e-cadherin protein levels were achieved by western blot.

miR-205 is involved in metastatic potential of 21T series, a breast cancer progression model