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The Simian Virus 40 (SV40) large T antigen is multifunctional protein with DNA helicase, RNA helicase and ATPse activities which contribute to multistep tumorogenesis in rodents and humans. The Immortomouse mouse strain expresses a mutated large T antigen tsA58 oncogene under the control of the interferon inducible murine H-2Kb promoter on chromosome 16. Our aim was to establish a BALB/c strain of H-2Kb-tsA58 immortomice that could be utilized to investigate specific pathological and physiological patterns associated SV-40 oncogenicity and generation of conditionally immortal cells lines.

Genotype and phenotype of balb/c mouse strain expressing h-2kb-tsa58- sv40 immortalizing oncogene