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Background Fluorescence lifetime of NADH had been proposed to use as an intrinsic biomarker for monitoring cellular metabolism. In our pervious studies, we have demonstrated that NADH lifetime of hMSCs increase gradually with time of osteogenic differentiation. In this study, we performed NADH lifetime measurement of hMSCs from a different donor and studied the association with several metabolic indices such as ATP level, oxygen consumption and lactate release. We also measured the quantity of Complex I, III, IV and V during hMSC differentiation.

bmc proceedings

The increase of NADH fluorescence lifetime is associated with the metabolic change during osteogenic differentiation of human mesenchymal stem cells (hMSCs)