English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Expression of recombinant antibodies in CHO cells is a state-of-the-art procedure in research and industry. Generation of cell lines producing high amounts of antibodies is one of the major tasks to increase process efficiency. The establishment of clones is often achieved by methotrexate (MTX)–mediated gene amplification in CHO-DG44 cells. Evaluation of MTX-mediated amplification is usually facilitated by intracellular staining of CHO cells.  We used the MACSQuant® Analyzer to determine the fraction of antibody-producing cells following staining of intracellular IgG with fluorochrome-conjugated antibodies. Additionally, the instrument was utilized for the rapid and reproducible determination of cell density and viability.

Determination of intracellular antibody production, cell density, and viability of recombinant CHO-DG44 cells using the MACSQuant® Analyzer