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Background Human cell lines are an interesting alternative to CHO cells for the production of recombinant proteins and monoclonal antibodies, because of their ability to produce genuine human posttranslational modifications. The human cell line AGE1.HN.AAT (ProBioGen, Berlin, Germany), that originated from human neural precursor tissue, has been adapted to serum-free conditions and cultivated in many different systems. Here we present our results using this cell line in a scale-up of batch cultivation from 50 mL vented polypropylene tube on a shaking platform, polycarbonate shakeflask (cultivation volume from 50 mL up to 300 mL), a 2 L-glass vessel stirred tank reactor and a 20 L-stainless steel stirred tank reactor (both Sartorius Stedim, Goettingen, Germany).

Characterisation of cultivation of the human cell line AGE1.HN.AAT