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Background In Systems Biology approaches a good amount of reliable data is essential for effective modelling of distinct biological processes. Hence the focus of the SysLogicsProject was on modelling of the central metabolism using different functional genomic techniques. For displaying the involved proteins, it is crucial to enrich them using subproteomic fractions. As many enzymes being involved in central metabolism are located in the mitochondria, we investigated the expression dynamics of mitochondrial proteins during batch cultivations of human AGE1.HN cells (ProBioGen, Berlin, Germany).

Analysis of the mitochondrial subproteome of the human cell line AGE1.HN – a contribution to a systems biology approach