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Background Metabolomics, aiming at the quantification of all extracellular and intracellular metabolites, is a valuable tool for characterizing, understanding and manipulating the physiology of mammalian cells. While extracellular metabolite analysis is well established, required quenching and extraction procedures for intracellular metabolite analysis in mammalian suspension cells are not yet routinely available. In this study a simple sampling and quenching protocol using ice-cold 0.9% saline as quenching solution [1] was tested on CHO cells. Quenching efficiency, preservation of cell integrity as well as cell separation and the necessity of washing steps were evaluated and possible sources of error are discussed.

Evaluation of sampling and quenching procedures for the analysis of intracellular metabolites in CHO suspension cells