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Background Mammalian cells are used extensively in the production of recombinant proteins, and of monoclonal antibodies (MAbs) in particular. The trend towards avoiding animal-derived components in biopharmaceutical production processes has led to the extensive use of nonanimal origin hydrolysates such as plant hydrolysates or yeast hydrolysates. The source of hydrolysates affects cell growth and productivity and may also affect product quality. Accordingly, careful consideration should be given during process and cell culture media development, in order to determine the appropriate type and amount of hydrolysates to be added, for the cell and product at hand. In this study, we assessed the impact of several hydrolysate additives and chemically defined (CD) commercial feeds on MAb titers, MAb average specific productivity (average Qp), cell viabilities and metabolite profiles in suspension cultures of recombinant CHO cells expressing a monoclonal antibody in shake flasks and 2 L bioreactors.

Medium and feed optimization for fed-batch production of a monoclonal antibody in CHO cells