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Background The promise of pluripotent stem cells lies in their ability to form any cell or tissue in the body. However, this promise requires a stable and reproducible method to grow the cells. Current methods rely on feeder cells or extracellular matrix proteins to cover the cultureware growth surface, and either manual selection or enzymatic dissociation in cell passaging and harvesting. This study describes a novel and simple method to grow pluripotent stem cells without the use of feeder cells or extracellular matrix proteins.

Innovative animal component-free surface for the cultivation of human embryonic stem cells