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The data presented illustrate how complex animal component free supplements may be employed to reduce or eliminate serum from many traditional vaccine production media, while providing equal or better performance with respect to cell growth. Partial or complete serum elimination was achieved in media used to cultivate CEF, MDCK and BHK-21 cell lines. These supplements have also proven to facilitate the transition from anchorage dependent to suspension culture.

Complex medium supplements optimized for the reduction of animal-derived components in vaccine production media