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BackgroundVero cells are adherent cell lines commonly used for theproduction of viral vaccines. We had developed an ani-mal component free medium that allows an optimalgrowth of this cell line in stirred bioreactor [1,2]. Wehad also showed that Vero cells grown in this medium(called IPT-AFM) sustained rabies virus replication, andresulted in an overall yield comparable to the levelobtained in serum-supplemented medium.IPT-AF medium contains plant hydrolysates, namelysoy (Hypep 1510) and wheat gluten hydrolysates(Hypeps 4601 and 4605). These peptones were shown topromote cell attachment and growth. However, althoughthese components are of non-animal origin, their use invaccine production process has several drawbacks,mainly due variability between lots.The aim of this work is to identify active peptidesfrom these hydrolysates that show a positive effect oncell adhesion, attachment and growth. For this purpose,the hydrolysates were fractionated using chromatogra-phy and precipitation techniques. The effect of the iso-lated fractions on Vero cells growth were tested in 24and 6-well cell culture plates using experimental designapproach. Fractions that sustain cell growth, werefurther tested in stirred culture on Cytodex1 microcar-riers, to confirm their positive effect on Vero cellsgrowth.Materials and methodsCell line: Vero cells adapted to IPT-AF medium asdescribed in Rourou et al. [2] were used in this study.Media: M199 medium was purchased from Invitro-gen, IPT-AFM was prepared as detailed in Rourou et al.[2]. Hypeps were provided by Sheffield Bio-Science.

Isolation of active peptides from plant hydrolysates that promote Vero cells growth in stirred cultures