English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

This work aims to characterize the dynamic metabolic responses of A549 lung tumor cells exposed to cisplatin (CDDP) and to 6 Gy ionizing radiation over a period of 48h. Control and CDDP/radiation treated cells, in the form of lysed suspensions, were directly analyzed by 1H High Resolution Magic Angle Spinning (HRMAS) NMR spectroscopy (500 MHz) and the changes in their intracellular metabolic profiles assessed by spectral integration and multivariate analysis. In this way, consistent variation patterns could be detected and specific metabolic effects related to drug and/or radiation exposure could be identified. In particular, significant time-dependent alterations were found in lipids and choline-containing compounds, as well as in low molecular weight metabolites such as some amino acids and nitrogenated bases. The results presented show that 1H NMR spectroscopy is a powerful tool for providing detailed biochemical information about the effects induced on cultured cells by external perturbations, such as a chemotherapy drug and ionizing radiation. In the future, this approach will be applied to lung cancer primary cultures subjected to different treatment regimens.

Metabolic responses of A549 lung cells to cisplatin and radiation exposure studied by 1H NMR spectroscopy