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Background  A label-free immunosensor from as-grown double wall carbon nanotubes (DW) bundles was developed for detecting  Salmonella typhimurium . The immunosensor was fabricated by using the as-grown DW bundles as an electrode material with an anti- Salmonella  impregnated on the surface. The immunosensor was electrochemically characterized by cyclic voltammetry. The working potential (100, 200, 300 and 400 mV  vs.  Ag/AgCl) and the anti- Salmonella  concentration (10, 25, 50, 75, and 100 μg/mL) at the electrode were subsequently optimized. Then, chronoamperometry was used with the optimum potential of 100 mV  vs.  Ag/AgCl) and the optimum impregnated anti- Salmonella  of 10 μg/mL to detect  S. typhimurium  cells (0-10 9  CFU/mL).    Results  The DW immunosensor exhibited a detection range of 10 2  to 10 7  CFU/mL for the bacteria with a limit of detection of 8.9 CFU/mL according to the IUPAC recommendation. The electrode also showed specificity to  S. typhimurium  but no current response to  Escherichia coli .    Conclusions  These findings suggest that the use of a label-free DW immunosensor is promising for detecting  S. typhimurium .

Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimuriumimmunoassay