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Methods B cells were loaded with purified “live” HHV-8, purified UV-light inactivated HHV-8 (UV-HHV-8) or soluble HHV-8 glycoprotein B (gB). HHV-8 replication was measured by real time PCR for viral DNA and intracellular staining (ICS) and flow cytometry for the viral lytic proteins ORF59 and K8.1. ICS was used to assess cellassociated, polyfunctional cytokine-chemokine production, and B cell supernatants were tested for cytokine/ chemokine secretion by Cytometric Bead Array (BD Biosciences).

Human herpesvirus 8 replicates in primary B lymphocytes and induces polyfunctional cytokine and chemokine responses