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Background  Most of breast cancers are considered sporadic and modulation of the two major genes  BRCA1  and  BRCA2  expressions caused by tissue-specific somatic mutations lead to this pathology. The nutritional intake of phytoestrogens seems to reduce the risk of breast cancer and investigation of their potential as anticancer agents has increased. However, the possible mechanisms and signalling pathways of phytoestrogen action in breast cancer prevention remains unknown.    Results  Using Taqman Low Density Array technology, we investigated the  BRCA2  loss of function role in sporadic breast cancers and the links existing with soy isoflavones on a panel of nuclear receptor expression. Human breast cell lines (MCF-7, MDA-MB-231, and MCF-10a) were transfected by  BRCA2 -siRNA and treated with genistein (18.5 μM) or daidzein (78.5 μM) for 72 h. Generating the transitory knockdown of  BRCA2  oncosuppressor, we observed different modulations in several nuclear receptor genes such as  ER ,  RAR  and  RXR , as well as  PPAR s and  VDR  according to the studied breast cell line. Additional isoflavone treatments showed different nuclear receptor gene modulation profiles.    Conclusion  Our results seemed to implicate the oncosuppressor  BRCA2  and the phytoestrogen pathways in different nuclear gene expressions  via  an ER-independent manner.

journal of molecular signaling

Expression analyses of nuclear receptor genes in breast cancer cell lines exposed to soy phytoestrogens after &lt;em&gt;BRCA2&lt;/em&gt; knockdown by TaqMan Low-Density Array (TLDA)