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Suspension cultures of  Catharanthus roseus  were used to evaluate ultraviolet-B (UV-B) treatment as an abiotic elicitor of secondary metabolites. A dispersed cell suspension culture from  C. roseus  leaves in late exponential phase and stationary phase were irradiated with UV-B for 5 min. The stationary phase cultures were more responsive to UV-B irradiation than late exponential phase cultures. Catharanthine and vindoline increased 3-fold and 12-fold, respectively, on treatment with a 5-min UV-B irradiation.

Enhanced catharanthine and vindoline production in suspension cultures of <em>Catharanthus roseus</em> by ultraviolet-B light