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Materials and methods The rTg4510 mice express the TauP301L mutation under control of a tetracycline-responsive operon promoter. rTg4510, single transgenic tTA and non-transgenic littermates (129S6/FVB/N F1 hybrid) were bred at Taconic, Denmark. Paraffin sections were stained for p-Tau, astroand microglial markers, ubiquitin, doublecortin and tangles. Western blot was performed on soluble (S1) and sarkosyl-insoluble (P3) brain fractions. Hippocampal CA1 neuron numbers and cortical neuronal densities were assessed by un-biased stereology at Gubra, Denmark.

Late neurodegeneration in the Tau transgenic mouse model rTg4510